Comparision of Polymerase Chain Reaction and IDEIA CHLAMYDIA in Detection of Chlamydia trachomatis from First-Voided Urine of Male Urethritis Patients
نویسندگان
چکیده
منابع مشابه
Diagnosis of Chlamydia trachomatis urethritis in men by polymerase chain reaction assay of first-catch urine.
To determine the accuracy of a recently developed polymerase chain reaction (PCR) urine assay to detect Chlamydia trachomatis urethral infection in men, we obtained urethral swabs and first-catch urine from 365 men attending a sexually transmitted diseases clinic. Thirty-three (9%) of the 365 men were infected with C. trachomatis as defined by urethral culture. Thirty-two of the 33 men with cul...
متن کاملDetection of Chlamydia trachomatis DNA in patients with non-gonococcal urethritis using the polymerase chain reaction.
A practical protocol using the polymerase chain reaction (PCR) was designed for detecting Chlamydia trachomatis in clinical samples. DNA was extracted from material collected on urethral swabs and used as substrate for the PCR. The target was a 600 basepair DNA segment of the multicopy plasmid that is common to all strains of the bacterium. Negative samples were checked for loss of DNA or prese...
متن کاملDetection of Chlamydia trachomatis Using polymerase chain reaction (PCR)
Chlamydia trachomatis now is one of the most prevalent bacteria sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. Diagnosis of Chlamydial infections is based on isolation of bacteria in tissue culture media that requires at least 48 to 72h. The aim of this study was the detection of Chlamydia trachomatis, using polymerase chain reaction (PCR), beca...
متن کاملDetection of Chlamydia trachomatis in urine samples by polymerase chain reaction and enzyme immunoassay.
First-void urine samples collected from sexually transmitted diseases (STD) clinic patients were examined by a nested polymerase chain reaction (PCR) and a commercial enzyme immunoassay (IDEIA Chlamydia) for the diagnosis of Chlamydia trachomatis urethritis or cervicitis. The primers for the PCR amplified a target in the major outer membrane protein (MOMP) gene in C trachomatis while the IDEIA ...
متن کاملDirect Detection of Chlamydia trachomatis in Urine
Screening for Chlamydia trachomatis infection in men has traditionally been limited to men who present with urethral symptoms, thereby limiting the detection of asymptomatic chlamydia infection in men. In order to effectively screen both symptomatic and asymptomatic men, we evaluated a newly developed polymerase chain reaction (PCR) assay, Amplicor C. trachomatis, from Roche Molecular Systems f...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 1992
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.66.1473